Paul R Johnston

Postdoc, Rolff Lab/Berlin Center for Genomics in Biodiversity.


I'm a postdoc in Jens Rolff's Evolutionary Biology group at the Free University of Berlin and the Berlin Center for Genomics in Biodiversity Research

I'm interested in host-microbe interactions especially the evolution of bacterial resistance to host defences, genomics of gut symbionts, and the consequences of dynamic developmental processes such as metamorphosis for symbiosis.


Postdoc, Freie Universität Berlin, 01/2011 - present.
"‘Multidrug’ resistance and the evolutionary ecology of insect immunity" with Jens Rolff (PI).
Funded by the European Research Council.
(University of Sheffield, 2011-2012)



Johnston, P. R., A. D. Dobson & J. Rolff, (2016). Genomic signatures of experimental adaptation to antimicrobial peptides in Staphylococcus aureus. G3: Genes, Genomes, Genetics, 6(6), 1535-1539. pdf

Makarova O., A. Rodriguez-Rojas, M. Eravci, C. Weise, A. J. Dobson, P. R. Johnston & J. Rolff, (2016). Antimicrobial defence and persistent infection in insects revisited. Phil. Trans. R. Soc. B., 371: 20150296. pdf

Benoit J. B., Z. N. Adelman, K. Reinhardt, A. Dolan, M. Poelchau, E. C. Jennings, E. M. Szuter, R. W. Hagan, H. Gujar, J. N. Shukla, F. Zhu, M. Mohan, D. R. Nelson, A. J. Rosendale, C. Derst, V. Resnik, S. Wernig, P. Menegazzi, C. Wegener, N. Peschel, J. M. Hendershot, W. Blenau, R. Predel, P. R. Johnston, P. Ioannidis, R. M. Waterhouse, R. Nauen, C. Schorn, M. Ott, F. Maiwald, J. S. Johnston, A. D. Gondhalekar, M. E. Scharf, B. F. Peterson, K. R. Raje, B. A. Hottel, D. Armisén, A. J. J. Crumière, P. N. Refki, M. E. Santos, E. Sghaier, S. Viala, A. Khila, S. Ahn, C. Childers, C. Lee, H. Lin, D. S. T. Hughes, E. J. Duncan, S. C. Murali, J. Qu, S. Dugan, S. L. Lee, H. Chao, H. Dinh, Y. Han, H. Doddapaneni, K. C. Worley, D. M. Muzny, D. Wheeler, K. A. Panfilio, I. M. V. Jentzsch, E. L. Vargo, W. Booth, M. Friedrich, M. T. Weirauch, M. A. E. Anderson, J. W. Jones, O. Mittapalli, C. Zhao, J. Zhou, J. D. Evans, G. M. Attardo, H. M. Robertson, E. M. Zdobnov, J. M. C. Ribeiro, R. A. Gibbs, J. H. Werren, S. R. Palli, C. Schal, S. Richards, (2016). Unique features of a global human ectoparasite identified through sequencing of the bed bug genome. Nature Communications, 4:10165. pdf

Honnen, A. C., P. R. Johnston & M. T. Monaghan, (2016). Sex-specific gene expression in the mosquito Culex pipiens f. molestus in response to artificial light at night. BMC Genomics, 17:22. pdf

Johnston, P. R. & J. Rolff, (2015). Host and symbiont jointly control gut microbiota during complete metamorphosis. PLOS Pathogens. 11(11): e1005246. pdf

Johnston, P. R., D. J. Mikolajewski & J. Rolff, (2015). Identification of viruses associated with larvae of the dragonfly Leucorrhinia dubia, and damselfly Coenagrion puella from RNA sequencing data. International Journal of Odonatology, 18(1) 81-88. pdf

Otti, O., P. R. Johnston, G. Horsburgh, J. Galindo & K. Reinhardt, (2015). Female transcriptomic response to male genetic and non-genetic ejaculate variation. Behavioral Ecology, 26(3) 681-688. pdf

Johnston, P. R., O. Makarova & J. Rolff, (2014). Inducible defenses stay up late: temporal patterns of immune gene expression in Tenebrio molitor. G3: Genes, Genomes, Genetics, 4(6) 947-955 pdf

Johnston, P. R. & J. Rolff, (2013). Immune- and wound-dependent differential gene expression in an ancient insect. Developmental & Comparative Immunology, 40(3-4) 320-324. pdf

Dobson, A. D., P. R. Johnston, A. Vilcinskas & J. Rolff, (2012). Identification of immunological expressed sequence tags in the mealworm beetle Tenebrio molitor. Journal of Insect Physiology, 58(12), 1556-1561. pdf

Arce, A. N., P. R. Johnston, P. T. Smiseth & D. Rozen, (2012). Mechanisms and fitness effects of antibacterial defences in a carrion beetle. Journal of Evolutionary Biology, 25(5), 930-937. pdf

Raymond, B., P. R. Johnston, C. Nielsen-LeRoux, D. Lereclus & N. Crickmore, (2010). Bacillus thuringiensis: an impotent pathogen? Trends in Microbiology, 18(5), 183-232. pdf

Raymond, B., P. R. Johnston, D. J. Wright, R. J. Ellis, N. Crickmore & M. B. Bonsall, (2009). A mid-gut microbiota is not required for the pathogenicity of Bacillus thuringiensis to diamondback moth larvae. Environmental Microbiology, 11(10), 2556-2563. pdf

Johnston, P. R. & N. Crickmore, (2009). Gut bacteria are not required for the insecticidal activity of Bacillus thuringiensis toward the tobacco hornworm, Manduca sexta. Applied and Environmental Microbiology, 75(15), 5094-5099. pdf