Paul R Johnston

Berlin Center for Genomics in Biodiversity.


I'm interested in host-microbe interactions especially the evolution of bacterial resistance to host defences, genomics of gut symbionts, and the consequences of dynamic developmental processes such as metamorphosis for symbiosis.


Berlin Center for Genomics in Biodiversity Research, 07/2016 - date.



He, S., Sieksmeyer, T., Banasiak, R., Johnston, P. R., McMahon, D. P., 2018. An expanded repertoire of immune genes in the cockroach Blatta orientalis as revealed by de novo transcriptome analysis. Under review.

Sieksmeyer, T., He, S., Johnston, P. R., Rodríguez-Rojas, A., Kuropka, B., Weise, C., McMahon, D. P., 2018. Eating in a losing cause: anorexia and macronutrient manipulation fail to boost cockroach immunity. Under review.

Bourne, E. C., Johnston, P. R., Funke, E. and Monaghan, M. T., 2018. Gene expression analysis of litter-associated fungi using RNA-seq. In press.

Bracamonte, S. E., Johnston, P. R., Knopf, K. and Monaghan, M. T. 2018. Experimental infection with Anguillicola crassus alters immune gene expression in both spleen and head kidney of the European eel (Anguilla anguilla). Marine Genomics. In press.

Lu, Y., Johnston, P. R., Dennis, S. R., Monaghan, M. T., Uwe, J., Spaak, P., Wolinska, J., 2018. Daphnia galeata responds to the exposure to ichthyosporean gut parasite by down-regulation of immunity and lipid metabolism. Accepted.

Makarova, O.*, Johnston, P. R.*, Rodriguez-Rojas, A., el-Shazely, B., Moreno-Morales, J. and Rolff, J., 2018. Genomic signature of experimental adaptation of Staphylococcus aureus to a natural combination of insect antimicrobial peptides. Accepted.

He, S., Johnston, P. R., Kuropka, B, Lokatis, S., Weise, C., Plarre, R.,Kunte, H. J. and McMahon, D. P., 2018. Termite soldiers contribute to social immunity by synthesizing potent oral secretions. Insect Molecular Biology. In press.

Zanchi, C., Johnston, P. R. and Rolff, J., 2017. Evolution of defence cocktails: antimicrobial peptide combinations reduce mortality and persistent infection. Molecular ecology, 26(19), pp.5334-5343.

Berdan, E.L., Finck, J., Johnston, P. R., Waurick, I., Mazzoni, C.J. and Mayer, F., 2017. Transcriptome profiling of ontogeny in the acridid grasshopper Chorthippus biguttulus. PloS one, 12(5), p.e0177367.

Makarova, O.*, Johnston, P. R.*, Walther, B., Rolff, J. and Roesler, U., 2017. Complete Genome Sequence of the Livestock-Associated Methicillin-Resistant Strain Staphylococcus aureus subsp. aureus 08S00974 (Sequence Type 398). Genome announcements, 5(19), pp.e00294-17.

Makarova, O.*, Johnston, P. R.*, Walther, B., Rolff, J. and Roesler, U., 2017. Complete Genome Sequence of the Disinfectant Susceptibility Testing Reference Strain Staphylococcus aureus subsp. aureus ATCC 6538. Genome Announcements, 5(19), pp.e00293-17.

Johnston, P. R.*, Dobson, A.J.* and Rolff, J., 2016. Genomic signatures of experimental adaptation to antimicrobial peptides in Staphylococcus aureus. G3: Genes, Genomes, Genetics, 6(6), pp.1535-1539.

Makarova, O., Rodriguez-Rojas, A., Eravci, M., Weise, C., Dobson, A., Johnston, P. R. and Rolff, J., 2016. Antimicrobial defence and persistent infection in insects revisited. Phil. Trans. R. Soc. B, 371(1695), p.20150296.

Benoit, J.B. et al., 2016. Unique features of a global human ectoparasite identified through sequencing of the bed bug genome. Nature communications, 7, p.ncomms10165.

Honnen, A.C.*, Johnston, P. R.* and Monaghan, M.T., 2016. Sex-specific gene expression in the mosquito Culex pipiens f. molestus in response to artificial light at night. BMC genomics, 17(1), p.22.

Johnston, P. R. and Rolff, J., 2015. Host and symbiont jointly control gut microbiota during complete metamorphosis. PLoS pathogens, 11(11), p.e1005246.

Otti, O., Johnston, P. R., Horsburgh, G.J., Galindo, J. and Reinhardt, K., 2014. Female transcriptomic response to male genetic and nongenetic ejaculate variation. Behavioral Ecology, 26(3), pp.681-688.

Johnston, P. R., Mikolajewski, D.J. and Rolff, J., 2015. Identification of viruses associated with larvae of the dragonfly Leucorrhinia dubia, and damselfly Coenagrion puella from RNA sequencing data. International journal of odonatology, 18(1), pp.81-88.

Johnston, P. R., Makarova, O. and Rolff, J., 2014. Inducible defenses stay up late: temporal patterns of immune gene expression in Tenebrio molitor. G3: Genes, Genomes, Genetics, 4(6), pp.947-955.

Johnston, P. R. and Rolff, J., 2013. Immune-and wound-dependent differential gene expression in an ancient insect. Developmental & Comparative Immunology, 40(3-4), pp.320-324.

Dobson, A.J., Johnston, P. R., Vilcinskas, A. and Rolff, J., 2012. Identification of immunological expressed sequence tags in the mealworm beetle Tenebrio molitor. Journal of insect physiology, 58(12), pp.1556-1561.

Arce, A.N., Johnston, P. R., Smiseth, P.T. and Rozen, D.E., 2012. Mechanisms and fitness effects of antibacterial defences in a carrion beetle. Journal of evolutionary biology, 25(5), pp.930-937.

Raymond, B., Johnston, P. R., Nielsen-LeRoux, C., Lereclus, D. and Crickmore, N., 2010. Bacillus thuringiensis: an impotent pathogen?. Trends in microbiology, 18(5), pp.189-194.

Raymond, B., Johnston, P. R., Wright, D.J., Ellis, R.J., Crickmore, N. and Bonsall, M.B., 2009. A mid‐gut microbiota is not required for the pathogenicity of Bacillus thuringiensis to diamondback moth larvae. Environmental microbiology, 11(10), pp.2556-2563.

Johnston, P. R. and Crickmore, N., 2009. Gut bacteria are not required for the insecticidal activity of Bacillus thuringiensis toward the tobacco hornworm, Manduca sexta. Applied and environmental microbiology, 75(15), pp.5094-5099.

*equal contribution